Zur Verwendung von 2‐Hydroxyethyl‐Methacrylat (GMA) als Einbettungsmedium bei histologischen Untersuchungen in der Phytopathologie

0303 health sciences 03 medical and health sciences
DOI: 10.1111/j.1439-0434.1985.tb04325.x Publication Date: 2008-05-01T17:07:01Z
ABSTRACT
Abstract The use of 2‐hydroxyethyl‐methacrylate (GMA) as embedding medium for histological investigations in phytopathology A new plastic technique is described subsequent thin sectioning plant tissues. In comparison to the paraffinmethod GMA polymerization system less time consuming. excellent preservation well‐fixed tissue fully asserted, not removed from sections. lightmicroscopic studies convincing results were obtained with different staining procedures; specific evidence polysaccharides, pectine and nucleic acids was carried out sections 2‐8 μm thickness, also by fluorescence microscopy. GMA‐embedding seems be value various phytopathology.
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