Characterization of Closterovirus‐like Particles Associated with Grapevine Leafroll Disease

• DOI: 10.1111/j.1439-0434.1990.tb04247.x
• Publication Date: 2008-05-01T16:23:58Z
• Authors: J. S. Hu; D. Gonsalves; D. Teliz
• Keywords: Closterovirus; Polyclonal antibodies; Ouchterlony double immunodiffusion

Abstract A purification procedure (Z ee et al. 1987) for closterovirus‐like particles associated with grapevine leafroll disease was improved after various sources of plant tissues, extraction buffers, pH values, and centrifugation techniques had been examined. An antiserum against the CA‐4 isolate produced used in ELISA. The virus‐like NY‐1 measured crude preparations were about 1,800 to 1,900 nm long. molecular weight coat protein NY‐1and NY‐2 isolates ca. 43 × 10 3 daltons SDS‐PAGE analysis; bands having this reacted Western blotting tests specific polyclonal monoclonal antibodies. large dsRNA molecule (ca. 6 Mr) along, lower species isolated from diseased grapevines, but not healthy grapevines. Seven tested their serological relatedness a A‐gold labelling immunosorbent electron microscopy, assay. Results indicated that serologically distinct serotypes existed, mixed infection grapevines different scrotypes common. grouping into serotype I, II, III, IV is proposed.