Characterization of Variability inRhizoctonia solaniby Using Morphological and Molecular Markers

• DOI: 10.1111/j.1439-0434.2005.01000.x
• Publication Date: 2005-07-22T02:10:21Z
• Authors: M. Sharma; S. K. Gupta; T. R. Sharma
• Keywords: DNA profiling; Primer (cosmetics); Genetic Variability

Abstract Twenty‐four isolates of Rhizoctonia solani (teleomorph: Thanatephorus cucumeris ) collected from soil, root and collar rot or foliage blight‐infected plants several locations north India were used for the analysis variability by using morphological molecular markers. Among characters, variation was observed in hyphal cell size. Seventeen produced few to abundant, white dark brown black, small large sclerotia generally middle colony. Genetic also analysed 11 random amplified polymorphic DNA primers (RAPD), four universal rice (URPs) two inter simple sequence repeat (ISSR) markers fingerprint patterns generated each isolate. The size bands ranged 0.3 3.5 kb all with RAPD, URP ISSR obtained same hosts geographical regions showed similarity profiles barring exceptions. All classified into groups A comparison data different that URPs are superior detecting genetic among R. . Hence, use URP's, which have long primer higher annealing temperature, would be more sensitive reliable characterizing diversity