Suitability of RAPD analysis for the detection of somaclonal variants in oil palm (Elaeis guineensis Jacq)

• DOI: 10.1111/j.1439-0523.1998.tb01451.x
• Publication Date: 2006-04-30T06:44:46Z
• Authors: A. Rival; L. Bertrand; T. Beulé; M.C. Combes; P. Trouslot; P. Lashermes
• Keywords: Elaeis guineensis; Somaclonal variation

Abstract Random amplified polymorphic DNA (RAPD) analysis using arbitrary 10‐mer oligonucleotide primers was employed in order to investigate the genetic fidelity of somatic embryogenesis‐derived regenerants oil palm ( Elaeis guineensis Jacq). Clonal palms bearing ‘mantled’ phenotype were identified field and ability RAPD markers distinguish these variants from normal type assessed. Of 387 used, 259 (67%) successfully used amplify genomic fragments with consistently reproducible banding. primers, 73 (19%) enabled identification polymorphism between clones. No intraclonal variability no differences mother could be total number scored (8900). Twenty‐four chosen for use a larger experiment aimed at comparing, first, genome that its clonal offspring and, second, true‐to‐type variant regenerants. Thus, regeneration protocol based on embryogenesis set up propagation does not induce any gross changes. The results obtained revealed however, approach is suitable detection phenotype. somaclonal variation discussed.