Transgenic cotton, expressing Amaranthus caudatus agglutinin, confers enhanced resistance to aphids

• DOI: 10.1111/j.1439-0523.2006.01247.x
• Publication Date: 2006-07-18T20:29:07Z
• Authors: J. Wu; X. Luo; H. Guo; J. Xiao; Y. Tian
• Keywords: Aphis gossypii; Cry1Ac

Abstract To evaluate the possible antiaphid function of Amaranthus caudatus agglutinin (ACA) in allogenetic plants, transgenic cotton plants expressing ACA gene under control a phloem‐specific promoter were generated via Agrobacterium ‐mediated transformation. Based on results Southern blot analyses, six with single or lower copy transgene and favourable agronomic traits selected for further studies. expression levels ranged from 0.02% to 0.45% total soluble protein as determined by Western analysis plants. Insect bioassays using nymphs aphid ( Aphis gossypii Glover) showed that five significantly inhibited population growth aphid, highest inhibition rate 64.5%. These shed some new light well promising application obtaining aphid‐resistant reduce yield loss honeydew contamination fibre aphids.