Misuse of Antimicrobials and Selection of Methicillin‐Resistant Staphylococcus pseudintermedius Strains in Breeding Kennels: Genetic Characterization of Bacteria After a Two‐year Interval

• DOI: 10.1111/j.1439-0531.2012.02012.x
• Publication Date: 2012-05-03T04:55:27Z
• Authors: A Rota; C Milani; M Corrò; I Drigo; S Börjesson
• Keywords: Staphylococcus pseudintermedius

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains have been isolated from dogs with increasing frequency; prolonged or excessive use of antimicrobials is associated the selection MRSP, and misuse frequent in breeding kennels. This study was carried out two kennels (A B) which we had MRSP 2008: aim to assess colonization previously positive bitches other sharing same environment genetic profile both old new [spa typing, staphylococcal cassette chromosome mec (SCCmec) typing Pulsed-field Gel Electrophoresis (PFGE)]. Six animals Kennel A (two 2008) eight B (one were tested: 16 only housed B. Old isolates mecA positive, resulted spa type t02 SSCmec II-III. PGFE showed that all related belonged main clone lineage dominating Europe, ST71-J-t02-II-III. Kennels differ antimicrobials, has reduced over time A, while remained B, where many agents belonging different classes (third-generation cephalosporins, fluoroquinolones, macrolids) are administered dogs, without veterinary supervision, especially around parturition. Misuse key factor for healthy their persistence time. Dog breeders should be aware infections caused by multiresistant bacteria very limited therapeutical options represent a huge challenge animal health.