C‐kit expression in spermatogonia damaged by doxorubicin exposure in mice

• DOI: 10.1111/j.1447-0756.2012.02006.x
• Publication Date: 2012-10-30T02:26:40Z
• Authors: Reiko Tanigaki; Kou Sueoka; Hiroto Tajima; Akira Nakabayashi; Kenji Sato; Hironori Asada; Shingo Kato; Yoshihiko Hosoi; Yasunori Yoshimura
• Keywords: Male; 0301 basic medicine; Antibiotics, Antineoplastic; Fertilization in Vitro; Organ Size; Immunohistochemistry; Spermatogonia; 3. Good health; Semen Analysis; Disease Models, Animal; Mice; Proto-Oncogene Proteins c-kit; 03 medical and health sciences; Doxorubicin; Testis; Animals; RNA, Messenger; Spermatogenesis; Infertility, Male

The purpose of this study was to assess the relationship between chronically impaired spermatogenesis induced by exposing mice doxorubicin (DXR) and expression infertility factor c-kit.Eight-week-old male Institute for Cancer Research (ICR) were intraperitoneally treated with DXR (0.15 mg/kg, group) or saline control twice weekly five weeks killed 14 after initial exposure. animals sacrificed bilateral testes removed weighed. stored mRNA assay fixed immunohistochemistry. Some testicular samples in 10% formalin histopathological examination.Testicular weight (67.6 ± 9.7 mg, P < 0.05), sperm motility (18 6.0%, 0.05) fertilization rate (2-to-16-cell embryos, 5%; significantly lower group than group. In there severe tissue damage from spermatogonia onward, Sertoli cell ratio (38% vs. 9%, 0.05). addition, a decrease c-kit protein expression, amount messenger ribonucleic acid (mRNA) according semiquantitative method also decreased.Expression long-term, low-dose administration correlated number spermatogonia.