Incomplete thermal ablation stimulates proliferation of residual renal carcinoma cells in a translational murine model

• DOI: 10.1111/j.1464-410x.2012.11261.x
• Publication Date: 2012-05-22T08:44:39Z
• Authors: Stephanie G.C. Kr...; Harm H.E. van Melick; Maarten W. Nijkamp; Fabian K. Kruse; Laura W.J. Kruijssen; Paul J. van Diest; J.L.H. Ruud Bosch; Judith J.M. Jans
• Keywords: Renal carcinoma; Thermal ablation

What's known on the subject? and What does study add? Thermal ablation influences local tissue microenvironment. Several studies have reported that residual tumour cells may exhibit a more aggressive phenotype. This shows incomplete CA RFA cause an increased proliferation decreased apptosis of renal cells. be caused by stimulatory factors such as hypoxia, HSPs inflammatory OBJECTIVE To compare effect thermal vs partial nephrectomy (PN) growth stimulation cellular survival in tumours. MATERIALS AND METHODS Renca tumours were transplanted under capsule mice (four to six mice/group) after which radiofrequency (RFA), cryoablation (CA) or PN was performed. At several time points treatment, presence cell proliferation, apoptosis, hypoxic areas, heat‐shock proteins (HSPs) 70 90 evaluated using immunohistochemistry. RESULTS 2 h showed proliferation. hyperproliferation significantly stronger than ( P < 0.05) not present PN. Residual apoptosis from days ablation. Apoptotic less evident at 3 0.001). Hypoxic areas increasingly up 7 Inflammatory infiltrated mainly necrotic ablation, their abundance peaked 1 week 0.05). The preceded hypoxia HSPs. CONCLUSIONS result factors, e.g. cells, could facilitate recurrences highlights importance achieving complete destruction.