Functional defect at the rice choline monooxygenase locus from an unusual post‐transcriptional processing is associated with the sequence elements of short‐direct repeats

• DOI: 10.1111/j.1469-8137.2007.02124.x
• Publication Date: 2007-06-07T11:50:06Z
• Authors: Di Luo; Xiangli Niu; Yuguo Wang; Wenjing Zheng; Lijuan Chang; Qilin Wang; Xin Wei; Guirong Yu; Bao‐Rong Lu; Yongsheng Liu
• Keywords: Betaine; Direct repeat; Coding region

• Glycine betaine (GB), a quaternary ammonium solute, plays crucial role in developing osmotic tolerance. Rice contains choline monooxygenase (CMO) and two aldehyde dehydrogenase homologues that are required for GB synthesis, but usually no is accumulated rice (Oryza sativa). To elucidate the molecular processes underlie deficiency rice, an experiment involving spinach (Spinacia oleracea) was conducted to analyze products transcribed from CMO genes. Reverse transcription-polymerase chain reaction (RT-PCR) used obtain transcripts sequencing approach employed structural composition of various transcripts. The results showed most were processed incorrectly, retaining introns or deleted coding sequences; unusual deletion events occurred at sequence elements short-direct repeats. In conclusion, production incorrect full-length protein probably reduces accumulation considerably plants. Sequence comparison also implied deletion-site selection might be mediated by repeats response stress conditions.