Prediction of pre‐eclampsia by an analysis of placenta‐derived cellular mRNA in the blood of pregnant women at 15–20 weeks of gestation

• DOI: 10.1111/j.1471-0528.2010.02491.x
• Publication Date: 2010-01-29T10:28:36Z
• Authors: A Sekizawa; Y Purwosunu; A Farina; H Shimizu; M Nakamura; N Wibowo; N Rizzo; T Okai
• Keywords: Univariate analysis

A panel of cellular mRNA markers was used to predict the occurrence pre-eclampsia in pregnant women at 15-20 weeks gestation.Prospective cohort study.The Department Obstetrics and Gynaecology, University Indonesia, Cipto Mangunkusumo National Hospital, Indonesia.Peripheral blood samples from asymptomatic women.Among 660 women, 62 developed later gestation (pre-eclampsia group) each case matched with five controls. Therefore, RNA expression levels component maternal were compared those 310 controls.The genes related angiogenesis oxidative stress between control groups. receiver operating characteristic (ROC) curve analyse sensitivity available marker. logistic regression analysis performed calculate odds for woman be classified as a case.The univariate ROC identified soluble vascular endothelial growth factor receptor-1 (Flt-1) endoglin (ENG) highest sensitivity. The best multivariate model obtained by combining Flt-1, ENG, placental (PlGF) parity. relative yielded 66% 10% 1 - specificity rate an area under 0.884 (P < 0.001).A can development long before clinical onset.