Developmental changes of glycosphingolipids and expression of glycogenes in mouse brains

• DOI: 10.1111/j.1471-4159.2007.04910.x
• Publication Date: 2007-09-22T00:02:59Z
• Authors: Sathaporn Ngamukote; Makoto Yanagisawa; Toshio Ariga; Susumu Ando; Robert K. Yu
• Keywords: Ganglioside; Nervous tissue

Abstract Glycosphingolipids (GSLs) and their sialic acid‐containing derivatives, gangliosides, are important cellular components abundant in the nervous system. They known to undergo dramatic changes during brain development. However, knowledge on mechanisms underlying qualitative is still fragmentary. In this investigation, we have provided a detailed study developmental of expression patterns GSLs, GM3, GM1, GD3, GD1a, GD2, GD1b, GT1b, GQ1b, A2B5 antigens (c‐series gangliosides such as GT3 GQ1c), Chol‐1α (GT1aα GQ1bα), glucosylceramide, galactosylceramide (O1 antigen), sulfatide (O4 stage‐specific embryonic antigen‐1 (Lewis x) glycolipids, human natural killer‐1 glycolipid (sulfoglucuronosyl paragloboside) developing mouse brains [embryonic day 12 (E12) adult]. E12–E14 brains, GD3 was predominant ganglioside. After E16, concentrations GM3 markedly decreased, a‐series increased. GT3, were expressed brains. Human transiently On other hand, Chol‐1α, galactosylceramide, exclusively found after birth. To provide better understanding metabolic basis for these changes, analyzed glycogene that GSL regulated primarily by glycosyltransferases, not glycosidases. parallel studies using primary neural precursor cells culture tool studying events, ganglioside glycosyltransferase gene also detected neurons induced differentiate from cells, including followed up‐regulation complex a‐ b‐series gangliosides. These cell systems resemble occurring brain. We conclude pattern content can serve useful markers development at level expression.