Role of vesicle pools in action potential pattern‐dependent dopamine overflow in rat striatum in vivo

• DOI: 10.1111/j.1471-4159.2011.07440.x
• Publication Date: 2011-08-20T10:40:17Z
• Authors: Shi‐Rong Wang; Wei Yao; Hong‐Ping Huang; Bo Zhang; Pan‐Li Zuo; Liang Sun; Hai‐Qiang Dou; Qing Li; Xin‐Jiang Kang; Hua‐Dong Xu; Mei‐Qin Hu; Mu Jin; Lei Zhang; Yu Mu; Ji‐Yun Peng; Claire Xi Zhang; Jiu‐Ping Ding; Bao‐Ming Li; Zhuan Zhou
• Keywords: Medial forebrain bundle

Action potential (AP) patterns and dopamine (DA) release are known to correlate with rewarding behaviors, but how codes of AP bursts translate into DA in vivo remains elusive. Here, a given pattern was defined by four codes, termed total number, frequency, number bursts, interburst time [N, f, b, i].. The 'burst effect' calculated the ratio (γ) overflow multiple that single burst when fixed. By stimulating medial forebrain bundle using at either physiological (20 Hz) or supraphysiological (80 frequencies, we found released from two kinetically distinct vesicle pools, fast-releasable pool (FRP) prolonged-releasable (PRP), striatal dopaminergic terminals vivo. We examined effects pools on AP-pattern dependent found, codes' [b=8, i=0.5 s], large [N = 768, f 80 Hz] produced facilitating burst-effect (γ[b8/b1] 126 ± 3%), while small [N=96, triggered depressing-burst-effect 29 4%). Furthermore, PRP (but not FRP) predominantly contributed facilitating-burst-effect FRP played an important role depressing-burst effect. Thus, our results suggest captures pre-synaptic information through different releasable pools.