Novel primers for detection and quantification of Myxococcus species in situ

• DOI: 10.1111/j.1471-8286.2006.01339.x
• Publication Date: 2006-07-20T10:06:06Z
• Authors: K. J. MARTIN; C. T. BULL
• Keywords: Myxococcus xanthus

Abstract A nested polymerase chain reaction (PCR) protocol using unique primers was developed to detect and quantify Myxococcus species from environmental samples. The amplified most of when 10 pg DNA representing 1000 cells present, although over half were with as little 0.1 (10 cells). did not amplify other myxobacterial species, members the δ‐proteobacteria or unrelated organisms tested at significantly higher concentrations DNA. also used in quantitative PCRs, which accurately estimated population levels soil.