Isolation and characterization of psychrophilic yeasts producing cold-adapted pectinolytic enzymes

• DOI: 10.1111/j.1472-765x.2004.01503.x
• Publication Date: 2004-04-02T07:22:09Z
• Authors: T. Nakagawa; T. Nagaoka; S. Taniguchi; T. Miyaji; N. Tomizuka
• Keywords: Psychrophile; Pectin; Pectinesterase

The present study was conducted to screen for psychrophilic yeasts that are able degrade pectin compounds at low temperature, and examine the cold-active pectinolytic enzymes produced by isolated yeasts.Psychrophilic yeasts, which grow on as a sole carbon source, pectinolytic-psychrophilic yeast (PPY) strains PPY-3, 4, 5 6, were from soil Abashiri (Hokkaido, Japan). sequences of 28S rDNA D1/D2 PPY-3 4 indicated taxonomic affiliation Cryptococcus cylindricus Mrakia frigida, respectively, PPY-5 6 belonged Cystofilobasidium capitatum. below degrees C, showed activities several enzymes.The findings this indicate possibility produce novel temperature.It is possible can be applied food industry, e.g. clarification fruit juice C.