Purification and molecular characterization of subtilisin-like alkaline protease BPP-A from Bacillus pumilus strain MS-1

• DOI: 10.1111/j.1472-765x.2005.01851.x
• Publication Date: 2006-01-30T17:11:50Z
• Authors: T. Miyaji; Y. Otta; T. Nakagawa; T. Watanabe; Y. Niimura; N. Tomizuka
• Keywords: Subtilisin; Bacillus pumilus; Bacillaceae; Strain (injury)

The present study was conducted by screening zein-degrading bacteria in an attempt to obtain protease.Soil were screened formation of a clear zone on zein plates. Characterization bacterium indicated taxonomic affiliation Bacillus pumilus, and named MS-1 strain. strain produced two different types extracellular proteases, BPP-A BPP-B. In this study, we purified characterized because it exhibited higher ability hydrolyze than When casein used as the substrate, optimal pH for 11.0. BPP-A, better substrate at 13.0, whereas one bppA gene encoded 383-amino acid pre-pro form mature contained 275 amino residues. It concluded that belonged subtilisin family.A assigned B. pumilus extreme alkaline condition.This is first report micro-organisms. subtilisin-like protease possible utilize industrial enzyme production hydrolysates.