INCORPORATION AND METABOLISM OF [14C]‐ARACHIDONIC ACID IN GUINEA‐PIG LUNGS

• DOI: 10.1111/j.1476-5381.1979.tb08697.x
• Publication Date: 2012-07-19T15:45:16Z
• Authors: P.J. JOSE; J.P. SEALE
• Keywords: Male; 0301 basic medicine; 0303 health sciences; Time Factors; Guinea Pigs; Arachidonic Acids; In Vitro Techniques; Bradykinin; Lipid Metabolism; 03 medical and health sciences; Prostaglandins; Animals; SRS-A; Lung

1 Following infusion of [(14)C]-arachidonic acid into guinea-pig isolated lungs more than half the administered radioactivity was retained by lung.2 The majority present in phospholipid fraction with lesser amounts neutral lipid and free fatty fractions. When methyl esters were prepared, 80% co-chromatographed arachidonate.3 Transformation to cyclo-oxygenase products subsequent emergence lung effluent accounted for approximately 20% infused radioactivity.4 After pretreatment acid, stimulation arachidonic metabolism injections partially purified slow-reacting substance anaphylaxis (SRS-A), bradykinin or antigen challenge released rabbit aorta contracting (RCS) prostaglandin-like substances (PGLS) but little radioactivity. Furthermore, repeated SRS-A similar RCS PGLS diminishing radioactivity.5 These data indicated that exogenous taken up incorporated phospholipids. However, this newly had not equilibrated pool activated SRS-A, conversion products.