The Potential Effect of Botulinum Toxin Type A on Human Dermal Fibroblasts: An In Vitro Study

• DOI: 10.1111/j.1524-4725.2012.02504.x
• Publication Date: 2012-06-28T13:23:28Z
• Authors: Sang-Ha Oh; Young Lee; Young-Joon Seo; Jeung-Hoon Lee; Jung D. Yang; Ho Y. Chung; Byung C. Cho
• Keywords: Analysis of Variance; Cell Survival; Fibroblasts; Collagen Type I; Peptide Fragments; 3. Good health; Collagen Type I, alpha 1 Chain; 03 medical and health sciences; 0302 clinical medicine; Matrix Metalloproteinase 9; Neuromuscular Agents; Humans; Matrix Metalloproteinase 2; Botulinum Toxins, Type A; Procollagen; Cell Proliferation

The use of botulinum toxin type A (BoNT) continues to expand. Some physicians have noted a face-lifting effect after intradermal injection BoNT, although the effects are controversial.To investigate in vitro BoNT on human dermal fibroblasts.The proliferation and toxic fibroblasts were measured. To understand mechanism collagen production fibroblasts, procollagen I carboxy-terminal peptide (PIP) was measured using enzyme-linked immunosorbent assay, monitored Western blotting. examine degradation, we evaluated matrix metalloproteinase (MMP) gelatin zymography.BoNT did not stimulate or show fibroblasts. Levels PIP increased significantly grown presence upregulated expression decreased some MMPs that prevent degradation.This study shows interesting degradation vitro. This research provides experimental background for remodeling tissues aged skin.