Low‐energy extracorporeal shock wave therapy enhances skin wound healing in diabetic mice: A critical role of endothelial nitric oxide synthase

• DOI: 10.1111/j.1524-475x.2012.00851.x
• Publication Date: 2012-10-30T17:11:23Z
• Authors: Denso Hayashi; Kazuyoshi Kawakami; Kenta Ito; Keiko Ishii; Hiromasa Tanno; Yoshimichi Imai; Emi Kanno; Ryoko Maruyama; Hiroaki Shimokawa; Masahiro Tachi
• Keywords: Mice, Knockout; Vascular Endothelial Growth Factor A; 0301 basic medicine; Wound Healing; Nitric Oxide Synthase Type III; Reverse Transcriptase Polymerase Chain Reaction; Microcirculation; Ultrasonic Therapy; Blotting, Western; Neovascularization, Physiologic; Immunohistochemistry; 3. Good health; Diabetes Mellitus, Experimental; Mice; 03 medical and health sciences; Mice, Inbred NOD; Skin Ulcer; Animals

Abstract Low‐energy extracorporeal shock wave ( LE ‐ ESW ) treatment has been shown to accelerate wound repair; however, the mechanisms of remain unclear. In present study, we addressed role endothelial nitric oxide synthase eNOS ). A single accelerated healing wounds in diabetic mice caused by injection streptozotocin. This was accompanied increased expression and vascular growth factor VEGF generation new vessels at tissues. These results raised possibility that may be involved beneficial effects treatment. To address this possibility, compared between with a genetic disruption knockout eNOS‐KO mice) wild‐type WT control mice. Interestingly, ‐induced acceleration closure increase neovascularization significantly attenuated Considered collectively, these showed induced tissues played critical therapeutic accelerating promoting neovascularization.