Vesicles generated during storage of red cells are rich in the lipid raft marker stomatin

• DOI: 10.1111/j.1537-2995.2007.01549.x
• Publication Date: 2008-02-23T08:08:43Z
• Authors: Ulrich Salzer; Rong Zhu; Marleen Luten; Hirotaka Isobe; Vassili Pastushenko; Thomas Perkmann; Peter Hinterdorfer; Giel J.C.G.M. Bosman
• Keywords: Raft; Lipid raft; Microvesicle

BACKGROUND: The release of vesicles by red blood cells (RBCs) occurs in vivo and vitro under various conditions. Vesiculation also takes place during RBC storage results the accumulation units. membrane protein composition storage‐associated has not been studied detail. characterization vesicular might hint at underlying mechanism changes general vesiculation process particular. STUDY DESIGN AND METHODS: Vesicles from RBCs that had stored for periods were isolated same units used to generate calcium‐induced microvesicles. These two vesicle types compared with respect their size atomic force microscopy, raft content detergent‐resistant (DRM) analysis, thrombogenic potential activity annexin V binding thrombin generation, respectively. RESULTS: microvesicles are similar size, activity, composition. major differences relative concentrations integral DRM proteins. In vesicles, stomatin is twofold enriched flotillin‐2 threefold depleted. CONCLUSION: data indicate a stomatin‐specific, raft‐based involved vesiculation. A model proposed considering raft‐stabilizing properties stomatin, low temperature favoring aggregation, previously reported cytoskeletal organization.