Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2

• DOI: 10.1111/j.1567-1364.2012.00790.x
• Publication Date: 2012-01-20T01:44:25Z
• Authors: António Pacheco; Gabriel Talaia; Joana Sá-Pessoa; Daniela Bessa; Maria José Gonçalves; Roxana Moreira; Sandra Paiva; Margarida Casal; Odília Queirós
• Keywords: Lactobacillus casei

We aimed to manipulate the metabolism of Saccharomyces cerevisiae produce lactic acid and search for potential influence transport across plasma membrane in this process. W303-1A is able use l-lactic but its production our laboratory has not previously been detected. When l-LDH gene from Lactobacillus casei was expressed S. isogenic mutants jen1∆, ady2∆ jen1∆ ady2∆, all strains were acid, higher titres achieved mutant strains. In constitutively expressing both LDH JEN1 or ADY2, a external concentration found when glucose present medium, exhausted, consumption more pronounced. These results demonstrate that expression monocarboxylate permeases influences production. Ady2 characterized as an acetate permease demonstrated additional role lactate uptake. Overall, we transporters Jen1 are modulators may well be used export yeast cells.