Intestinal cytokines in children with pervasive developmental disorders

• DOI: 10.1111/j.1572-0241.2003.07593.x
• Publication Date: 2004-03-22T14:55:53Z
• Authors: Magee L. DeFelice; Eduardo D. Ruchelli; Jonathan E. Marko...; Melissa Strogatz; Krishna P. Reddy; Khadijeh Kadivar; Andrew E. Mulberg; Kurt A. Brown
• Keywords: Interquartile range; Histology; Proinflammatory cytokine

A relationship between autism and gastrointestinal (GI) immune dysregulation has been postulated based on incidence of GI complaints as well macroscopically observed lymphonodular hyperplasia microscopically determined enterocolitis in pediatric patients with autism. To evaluate immunity, we quantitatively assessed levels proinflammatory cytokines, interleukin (IL)-6, IL-8, IL-1beta, produced by intestinal biopsies children pervasive developmental disorders.Fifteen patients, six disorders nine age-matched controls, presenting for diagnostic colonoscopy were enrolled. Endoscopic organ cultured, supernatants harvested, IL-6, IL-1beta quantified ELISA. Tissue histology was evaluated blinded pathologists.Concentrations IL-6 from culture (median 318.5 pg/ml, interquartile range 282.0-393.0 pg/ml) when compared controls 436.9 312.6-602.5 not significantly different (p = 0.0987). Concentrations IL-8 84,000 16,000-143,000 177,000 114,000-244,000 0.0707). 0.0 0.0-94.7 0.0-60.2 0.8826). nonpathological all patients.We have demonstrated no significant difference production controls. In general, lower than These data fail to support an association inflammation.