Analysis of the Fecal Microbiota of Irritable Bowel Syndrome Patients and Healthy Controls with Real-Time PCR

• DOI: 10.1111/j.1572-0241.2005.40312.x
• Publication Date: 2005-01-24T13:23:58Z
• Authors: Erja Malinen; Teemu Rinttila; Kajsa Kajander; Jaana Matto; Anna Kassinen; Lotta Krogius; Maria Saarela; Riitta Korpela; Airi Palva
• Keywords: irritable bowel syndrome; Adult; DNA, Bacterial; Male; 0303 health sciences; fecal microbiota; gut microbiota; Bacteria; gut microflora; Sequence Analysis, DNA; Middle Aged; Polymerase Chain Reaction; 3. Good health; Irritable Bowel Syndrome; Feces; 03 medical and health sciences; IBS; gastrointestinal (GI) microbiota; Humans; Female; Aged

The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, microbiotas patients suffering from IBS were compared with a control group devoid gastrointestinal (GI) symptoms.Fecal (n = 27) fulfilling Rome II criteria for was age- gender-matched subjects 22). Fecal samples obtained at 3 months intervals. Total bacterial DNA analyzed by 20 quantitative real-time PCR assays covering approximately 300 species.Extensive individual variation observed in GI among both IBS- groups. Sorting according symptom subtypes (diarrhea, constipation, alternating predominant type) revealed that lower amounts Lactobacillus spp. present diarrhea whereas constipation carried increased Veillonella Average results three fecal suggested differences Clostridium coccoides subgroup Bifidobacterium catenulatum between 21) controls 15). Of intestinal pathogens earlier associated IBS, no indications Helicobacter or difficile found one case Campylobacter jejuni identified sequencing.With these assays, alterations found. Increasing microbial sequence information will further allow designing new more extensive analysis microbes IBS.