Interleukin-8 Regulates Expression of Reg Protein in Helicobacter pylori-Infected Gastric Mucosa

• DOI: 10.1111/j.1572-0241.2005.41915.x
• Publication Date: 2005-09-23T13:17:59Z
• Authors: Nagisa Yoshino; Shunji Ishihara; M.A.K. Rumi; C.F. Ortega-Cava; Takafumi Yuki; Hideaki Kazumori; Shin Takazawa; Hiroshi Okamoto; Yasunori Kadowaki; Yoshikazu Kinoshita
• Keywords: Interleukin 8; Enterochromaffin-like cell

Chronic inflammation induced by Helicobacter pylori infection is closely associated with epithelial cell proliferation and apoptosis, which are related to cellular turnover in gastric mucosa. Reg protein a regenerating gene product potent growth factor for mucosal cells, however, little known regarding its association the pathogenesis of H. infection. The aim this study was investigate production regulation pylori-associated gastritis.Gastric fundic biopsy samples were taken from patients without In vivo expression examined Western blotting immunohistochemistry methods. effects interleukin (IL)-8 on transcriptional activation ECC10 cells investigated luciferase assays, respectively.Reg found localized deeper part glands clearly shown chromogranin A-positive corpus. Semiquantitative results significantly polymorphonuclear neutrophil activity chronic IL-8 mucosa augmented infection, while dose-dependently stimulated promoter vitro cultured cells.The present showed first time that may be stimulator pylori-infected human IL-8. Further, our findings provide evidence novel link between help explain molecular mechanisms underlying diseases, including cancer.