Aminoguanidine renders inducible nitric oxide synthase knockout mice more susceptible toSalmonella typhimuriuminfection

• DOI: 10.1111/j.1574-6968.2002.tb10992.x
• Publication Date: 2006-01-09T14:27:30Z
• Authors: Xin Zhou; Douglas A. Potoka; Patricia Boyle; Evan P. Nadler; Kathleen McGinnis; Henri R. Ford
• Keywords: Mice, Knockout; Salmonella typhimurium; Salmonella Infections, Animal; 0303 health sciences; Nitric Oxide Synthase Type II; Nitric Oxide; Guanidines; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Macrophages, Peritoneal; Animals; Enzyme Inhibitors; Nitric Oxide Synthase

Aminoguanidine (AG), a nitric oxide synthase (NOS) inhibitor, has been widely used to study the role of inducible NOS (iNOS) in host defense against infections caused by various pathogens including Salmonella typhimurium. iNOS has been reported to play an important role in host defense against S. typhimurium infection both in vitro and in vivo. In this report we show those AG treatment lead to weight loss in both wild-type and iNOS knockout mice, and rendered them more susceptible to Salmonella infection. These results suggest that AG may have side effects other than the inhibition of iNOS, and that data obtained from studies using AG should be interpreted with caution.