Isolation and characterization of theRhodococcus opacusthiostrepton-inducible genestipALandtipAS: application for recombinant protein expression inRhodococcus

• DOI: 10.1111/j.1574-6968.2004.tb09675.x
• Publication Date: 2006-01-09T08:33:03Z
• Authors: Li Dong; Nobutaka Nakashima; Noriko Tamura; Tomohiro Tamura
• Keywords: Thiostrepton; Transcription

We cloned the Rhodococcus opacus (strain DSM 44193) tipA gene, which encodes two translation products, TipAL and TipAS. The gene products are homologous to Streptomyces spp. TipAS proteins, respectively. promoter is highly active protein predominantly accumulated in R. cells when inducer of transcription, thiostrepton, was presented culture medium. found that thiostrepton also induced expression an endogenous TipA-family erythropolis JCM3201). minimal region defined (57 bp) conserved nucleotide sequence putative binding site (TipA-box) identified region. presumed be transcribed into a leaderless mRNA. applied successfully for recombinant cells.