Ovine forestomach matrix biomaterial is a broad spectrum inhibitor of matrix metalloproteinases and neutrophil elastase

• DOI: 10.1111/j.1742-481x.2012.01106.x
• Publication Date: 2012-11-01T10:16:14Z
• Authors: Leonardo Negron; Stan Lun; Barnaby CH May
• Keywords: Neutrophil elastase; Proteolysis; Matrix (chemical analysis); Pancreatic elastase; Proteolytic enzymes

Proteases play a critical role in the ordered remodelling of extracellular matrix (ECM) components during wound healing and tissue regeneration. However, usually proteolysis is compromised chronic wounds due to over-expression high concentrations metalloproteinase's (MMPs) neutrophil elastase (NE). Ovine forestomach (OFM) decellularised matrix-based biomaterial developed for regeneration applications, including treatment wounds, heterogeneous mixture ECM proteins proteoglycans that retains native structural functional characteristics ECM. Given diverse molecular species present OFM, we hypothesised OFM may contain or fragments inhibit MMP NE activity. An extract was shown be potent inhibitor range MMPs (IC50 s = 23 ± 5 115 14 µg/ml) 157 37 µg/ml), more than extracts prepared from known protease modulating dressing. The broad spectrum activity against different classes (i.e. collagenases, gelatinases stromelysins) provide clinical advantage by effectively addressing imbalance seen wounds.