Comparison of Nuclear Morphometric Parameters in Cytologic Smears and Histologic Sections of Spontaneous Canine Tumors

• DOI: 10.1111/j.1939-165x.2002.tb00271.x
• Publication Date: 2008-03-05T10:47:58Z
• Authors: G. De Vico; A. Sfacteria; P. Maiolino; G. Mazzullo
• Keywords: Perimeter; Histology

Background — Nuclear morphometry may provide useful diagnostic and prognostic information for neoplasms in animals. Most available data have been obtained from histologic sections. of cytologic smears important pre‐operative information. Objectives The goal this study was to compare nuclear morphometric parameters sections spontaneous canine tumors. Methods Mean area (MNA), mean perimeter (MNP), form factor (FF; 2 /4π area) their respective SDs were assessed by image analysis both hematoxylin eosin‐stained May‐Grünwald‐Giemsa‐stained the same case 20 tumors different histogenesis. above selected as being best tools measuring variation shape size cells after neoplastic transformation. Data compared ANOVA with P<.01 considered significant. Results There a significant difference between specimens MNA, MNP, SDs. Only differences FF SD not statistically Conclusions related variability are comparable specimens. be affected fixation smear preparation techniques used histology cytology.