Polymerase Chain Reaction (PCR) Amplification of Parvoviral DNA from the Brains of Dogs and Cats with Cerebellar Hypoplasia

• DOI: 10.1111/j.1939-1676.2003.tb02475.x
• Publication Date: 2010-03-11T05:28:15Z
• Authors: Scott J. Schatzberg; Nicholas J. Haley; Stephen C. Barr; Colin Parrish; Samantha Steingold; Brian A. Summers; Alexander Lahunta; Joe N. Kornegay; Nicholas J.H. Sharp
• Keywords: Cerebellar hypoplasia (non-human); Hydranencephaly

Cerebellar hypoplasia in cats is caused most commonly by an utero or perinatal infection with feline panleukopenia virus (parvovirus). has been reported infrequently dogs, but no viral etiology identified to date. DNA was extracted from archival, paraffin-embedded, cerebellar tissue 8 and 2 canine littermates hypoplasia, cortical abiotrophy, 6 dogs congenital vermal defects, 1 dog hydranencephaly, 15 various encephalitides. The each cerebellum subject polymerase chain reaction (PCR) amplification 3 primer pairs specific for parvovirus DNA. Sequence analysis of PCR products the confirmed their identity parvoviral all control samples were negative parvovirus. Parvoviral structural proteins not immunohistochemistry either hypoplasia. This study shows that can be amplified archival brain might associated infection. Key words: Abiotrophy; Canine; Cerebellum; Dandy-Walker Syndrome; Feline; Parvovirus.