Experimental infection of ponies with equine influenza (H3N8) viruses by intranasal inoculation or exposure to aerosols

• DOI: 10.1111/j.2042-3306.1990.tb04217.x
• Publication Date: 2010-04-23T16:35:29Z
• Authors: JENNIFER A. MUMFORD; D. HANNANT; D. M. JESSETT
• Keywords: Equine influenza; Anorexia

Summary Infection of seronegative Welsh mountain ponies was established by intranasal instillation or exposure to nebulised aerosol egg grown H3N8 viruses. Pyrexia and coughing were noted following high titres virus recovered from the nasopharynx. Exposure resulted in more severe clinical signs characterised temperatures, dyspnoea, anorexia coughing; lower levels The severity kinetics shedding dose‐related with minimal infectious dose being 10 2 EID 50 /ml when exposed aerosols produced nebulisation 20ml allantoic fluid. Full only developed a 6 EIU /ml. It concluded that viruses reliable method inducing influenza than inoculation would be suitable for challenge studies.