Diagnosis of Equid herpesviruses −1 and −4 by polymerase chain reaction

• DOI: 10.1111/j.2042-3306.1992.tb02772.x
• Publication Date: 2010-04-23T17:08:09Z
• Authors: P. C. SHARMA; A. A. CULLINANE; D. E. ONIONS; L. NICOLSON
• Keywords: Base Sequence; Molecular Sequence Data; Nucleic Acid Hybridization; Herpesviridae Infections; 04 agricultural and veterinary sciences; Polymerase Chain Reaction; Sensitivity and Specificity; 3. Good health; 0403 veterinary science; Nasopharynx; DNA, Viral; Animals; Horse Diseases; Horses; Oligonucleotide Probes; Herpesviridae; Herpesvirus 1, Equid

Summary The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the detection Equid herpesviruses‐1 and −4 (EHV‐1 EHV‐4) within nasopharyngeal swab samples from horses. Ninety‐eight suspected field cases in‐contact horses were analysed. assays conducted blind later decoded compared with virus isolation data. Our results indicate that PCR rapid for diagnosis EHV‐1 EHV‐4 infection.