Presence of acyl‐homoserine lactones in 57 members of theVibrionaceaefamily

• DOI: 10.1111/jam.12264
• Publication Date: 2013-06-01T06:51:54Z
• Authors: A.A. Purohit; J. A. Johansen; H. Hansen; H.‐K.S. Leiros; A. Kashulin; C. Karlsen; A. Smalås; P. Haugen; N.P. Willassen
• Keywords: Photobacterium; Homoserine

The aim of this study was to use a sensitive method screen and quantify 57 Vibrionaceae strains for the production acyl-homoserine lactones (AHLs) map resulting AHL profiles onto host phylogeny.We used high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) protocol measure AHLs in spent media after bacterial growth. First, presence/absence (qualitative analysis) measured choose internal standard subsequent quantitative measurements. We screened from three genera (Aliivibrio, Photobacterium Vibrio) same family (i.e. Vibrionaceae). Our results show that about half isolates produced multiple AHLs, typically at 25-5000 nmol l(-1) .This work shows quorum sensing signals is found widespread among bacteria closely related produce similar profiles.The detection presented can be applied broad range samples may contribute wider mapping bacteria, example, clinically relevant strains.