Zinc toxicity and ATP production inPseudomonas fluorescens

• DOI: 10.1111/jam.12497
• Publication Date: 2014-03-13T18:08:38Z
• Authors: A. Alhasawi; C. Auger; V.P. Appanna; M. Chahma; V.D. Appanna
• Keywords: Pseudomonas fluorescens; Pseudomonadales; Zinc toxicity

Aims To identify the molecular networks in Pseudomonas fluorescens that convey resistance to toxic concentrations of Zn, a common pollutant and hazard biological systems. Methods Results strain ATCC 13525 was cultured growth medium with millimolar Zn. Enzymatic activities metabolite levels were monitored aid in-gel activity assays high-performance liquid chromatography, respectively. As oxidative phosphorylation rendered ineffective, assimilation citric acid mediated sequentially by citrate lyase (CL), phosphoenolpyruvate carboxylase (PEPC) pyruvate phosphate dikinase (PPDK) appeared play key role ATP synthesis via substrate-level (SLP). Enzymes generating antioxidant, reduced nicotinamide adenine dinucleotide (NADPH) enhanced, while metabolic modules mediating formation pro-oxidant, (NADH) downregulated. Conclusions reengineers its generate SLP, stratagem allows microbe compensate for an ineffective electron transport chain provoked excess Significance Impact Study The insights described here are critical devising strategies bioremediate Zn-polluted environments.