Antimicrobial and disinfectant resistance of Escherichia coli isolated from giant pandas

• DOI: 10.1111/jam.12820
• Publication Date: 2015-04-04T08:13:15Z
• Authors: L. Guo; M. Long; Y. Huang; G. Wu; W. Deng; X. Yang; B. Li; Y. Meng; L. Cheng; L. Fan; H. Zhang; L. Zou
• Keywords: Disinfectant; Agar dilution; Ailuropoda melanoleuca

The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). Antimicrobial testing was performed according standard disk diffusion method. minimal inhibitory concentrations (MICs) disinfectants were determined using agar dilution All screened for presence genes further analysed genetic relatedness by pulse‐field gel electrophoresis (PFGE). Results showed that 46·6% resistant at least one antimicrobial. fewer antimicrobials as panda age increased. Among antimicrobial‐resistant E. isolates, blaCTX‐M (88·2%) sul1 (92·3%) most prevalent. emrE, ydgE/ydgF, mdfA sugE(c) commonly present (68·2–98·9%), whereas qac sugE(p) relatively less prevalent (0–21·3%). frequencies tended be higher in isolated December than July, PFGE profiles also more diverse December. qacEΔ1 adolescent any other groups. revealed correlated well with sampling time habitat. This demonstrated common panda‐derived coli, associated could serve a critical vector spreading resistance. is first phenotypic characterizations 60 pandas. Frequent transfer cages may lead dissemination highlights need regularly monitoring bacteria