Inhibition ofListeria monocytogenesbiofilms by bacteriocin-producing bacteria isolated from mushroom substrate

• DOI: 10.1111/jam.13337
• Publication Date: 2016-12-12T05:06:42Z
• Authors: A.S. Bolocan; V. Pennone; P.M. O'Connor; A. Coffey; A.I. Nicolau; O. McAuliffe; K. Jordan
• Keywords: 2. Zero hunger; 0301 basic medicine; 03 medical and health sciences; Bacteria; Bacteriocins; Biofilms; Agaricales; Listeria monocytogenes

This study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate prevent biofilm formation by Listeria monocytogenes or remove existing biofilms in production facilities.It is generally recognized that L. forms can facilitate its survival food-processing environments. Eleven bacteriocin-producing isolates were identified and bacteriocins characterized based on heat enzyme inactivation studies. Further characterization undertaken MALDI-TOF mass spectrometry, PCR sequencing. Production nisin Z (by Lactococcus lactis isolates), subtilomycin Bacillus subtilis isolates) lichenicidin licheniformis sonorensis detected. In co-culture with monocytogenes, strains could reduce pre-formed biofilms.Mushroom be a source antagonize monocytogenes.The results highlight potential food environments, contributing reduction risk contamination environment.