Bactericidal activity of gallium-doped chitosan coatings against staphylococcal infection

• DOI: 10.1111/jam.14133
• Publication Date: 2018-10-17T13:42:22Z
• Authors: A. Ghalayani Esfa...; B. Lazazzera; L. Draghi; S. Farè; R. Chiesa; L. De Nardo; F. Billi
• Keywords: 0301 basic medicine; Staphylococcus aureus; Biomedical and clinical sciences; Clinical Sciences; Gallium; pulsed electromagnetic field; Microbiology; biofilm; 03 medical and health sciences; 0302 clinical medicine; veterinary and food sciences; Staphylococcus epidermidis; Humans; gallium; Agricultural; Chitosan; Biomedical and Clinical Sciences; Biological Sciences; Staphylococcal Infections; Anti-Bacterial Agents; 3. Good health; electrophoretic deposition; Biological sciences; Infectious Diseases; Emerging Infectious Diseases; biofilm; chitosan; electrophoretic deposition; gallium; postarthroplasty infection; pulsed electromagnetic field; Staphylococcus aureus; Staphylococcus epidermidis; Anti-Bacterial Agents; Biofilms; Chitosan; Gallium; Humans; Staphylococcal Infections; Staphylococcus epidermidis; Biofilms; postarthroplasty infection; Antimicrobial Resistance; chitosan; Infection

The aim of this study was to develop a new class gallium (Ga)-doped chitosan (CS) coatings fabricated by electrophoretic deposition (EPD) in staphylococcal infection therapy.Biofilm formation on EPD CS/Ga Staphylococcus epidermidis and aureus, which are the main strains involved postarthroplasty infections, assessed. codeposition an antibacterial agent effective; Ga loaded into CS matrix reduces biofilm viability up 86% 80% for S. aureus respectively. Lastly, influence pulsed electromagnetic field (PEMF) bactericidal activity investigated vitro. To end, were incubated with exposed PEMF using two different frequencies times. Biofilm decreased 35-40% presence low-frequency (LF) high-frequency (HF) not further reduced LF PEMF, but 38% at HF PEMF.This has established that combination PEMFs improves against strain 14990 12600.This integrated approach could reduce incidence orthopaedic implant applications. It also clearly demonstrates treatment aid biofilm-associated therapy due improved efficiency.