Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

• DOI: 10.1111/jam.14373
• Publication Date: 2019-07-09T07:06:29Z
• Authors: R. Filippousi; D. Antoniou; P. Tryfinopoulou; A.A. Nisiotou; G.‐J. Nychas; A.A. Koutinas; S. Papanikolaou
• Keywords: Isolation; 1,3-Propanediol

To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds.Ten Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, from fishes, metabolized Gly under nitrogen limitation. The aim study was identify potential microbial candidates that could produce single-cell oil (SCO), endopolysaccharides polyols when these micro-organisms were grown on Gly. As controls producing SCO glutinis NRRL YB-252 Cryptococcus curvatus Y-1511. At initial (Gly0 ) ≈40 g l-1 , most presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica produced non-negligible quantities mannitol arabitol (Ara). Five further cultivated at increasing Gly0 concentrations. 7·2 lipid (lipid DCW value ≈38% w/w), FMCC Y69 batch-bioreactor experiment with ≈80 30-33 ~30 Ara. shake-flasks ≈125 Ara ~48 (conversion yield polyol consumed ≈0·62 g-1 achieved. Cellular lipids all yeasts contained variable concentrations oleic, palmitic, stearic linoleic acids.Newly isolated, food-derived non-previously studied isolates converted several metabolites.Alternative ways crude valorization through fermentations provided compounds synthesized.