Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti‐biofilm and anti‐infection agent exopolysaccharide EPS273

• DOI: 10.1111/jam.14764
• Publication Date: 2020-07-03T14:21:07Z
• Authors: Z. Wu; R. Zheng; J. Zhang; S. Wu
• Keywords: Swarming motility

Relatively, few anti‐biofilm polysaccharides against Pseudomonas aeruginosa were done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection of P. aeruginosa. This study aims its anti‐infection mechanism on transcriptional level. Herein, we used an RNA‐Seq transcriptomic approach EPS273. The expression levels a large number genes changed after PAO1 was treated with Especially, related formation, such as gene involved in production extracellular matrix virulence factor, flagella cell motility iron acquisition. Notably, regulatory signal transduction markedly downregulated, two‐component system PhoP‐PhoQ quorum sensing (QS) LasI/LasR RhlI/RhlR. Furthermore, when phoP phoQ disrupted, respectively, reduction mutant △phoP or △phoQ also detected. may exert function by downregulating QS systems RhlI/RhlR aeruginosa, which further regulated formation. Our data will expand understanding mechanisms