Phospholipase C (AoPLC2) regulates mycelial development, trap morphogenesis, and pathogenicity of the nematode-trapping fungus Arthrobotrys oligospora

• DOI: 10.1111/jam.15370
• Publication Date: 2021-11-19T10:08:05Z
• Authors: Meihua Xie; Ni Ma; Na Bai; Meichen Zhu; Ke-Qin Zhang; Jinkui Yang
• Keywords: Conidiation

Phospholipase C (PLC) is a hydrolase involved in signal transduction eukaryotic cells. This study aimed to understand the function of PLC nematode-trapping fungus Arthrobotrys oligospora.Orthologous (AoPLC2) A. oligospora was functionally analysed using gene disruption and multi-phenotypic analysis. Disrupting Aoplc2 caused deformation partial hyphal cells (about 10%) conidia 50%), decreased number nuclei both cells, increased accumulation lipid droplets. Meanwhile, sporulation-related genes fluG abaA were downregulated ΔAoplc2 mutants than wild-type strain. Moreover, more sensitive osmotic stressors. Importantly, traps, electron-dense bodies nematicidal activity reduced, shape traps deformed. In addition, AoPLC2 biosynthesis secondary metabolites oligospora.AoPLC2 plays an important role development hyphae, spores, cell nuclei, responses stress, formation predation nematodes oligospora.This reveals various functions phospholipase elucidates regulation trap morphogenesis fungi.