Genome‐wide detection of structural variation in some sheep breeds using whole‐genome long‐read sequencing data

• DOI: 10.1111/jbg.12846
• Publication Date: 2024-01-22T07:15:00Z
• Authors: Guoyan Qiao; Pan Xu; Tingting Guo; Xue He; Yaojing Yue; Bohui Yang
• Keywords: Structural Variation

Abstract Genomic structural variants (SVs) constitute a significant proportion of genetic variation in the genome. The rapid development long‐reads sequencing has facilitated detection long‐fragment SVs. There is no published study to detect SVs using long‐read data from sheep. We applied mapping approach and characterized total 30,771 insertions, deletions, inversions translocations. identified 716, 916, 842 303 specific Southdown sheep, Alpine merino Qilian White Tibetan sheep Oula respectively. annotated these found that SV‐related genes were primarily enriched well‐established pathways involved regulation immune system, growth environmental adaptability. detected based on NGS resequencing validate accuracy third‐generation detection. Moreover, five candidate verified PCR method 50 Our first use construct novel map have completed preliminary exploration potential effects