The tumourigenicity of iPS cells and their differentiated derivates

• DOI: 10.1111/jcmm.12062
• Publication Date: 2013-05-27T04:16:45Z
• Authors: Zhiqiang Liu; Yu Tang; Shuanghong Lü; Jin Zhou; Zhiyan Du; Cuimi Duan; Zhiyan Li; Changyong Wang
• Keywords: Regenerative Medicine; Bioluminescence imaging

Induced pluripotent stem cell (iPSC) provides a promising seeding for regenerative medicine. However, iPSC has the potential to form teratomas after transplantation. Therefore, it is necessary evaluate tumorigenic risks of and all its differentiated derivates prior use in clinical setting. Here, murine iPSCs were transduced with dual reporter gene consisting monomeric red fluorescent protein (mRFP) firefly luciferase (Fluc). Undifferentiated iPSCs, from induced differentiation (iPSC-derivates), iPSC-derivated cardiomyocyte (iPSC-CMs) subcutaneously injected into back nude mice. Non-invasive bioluminescence imaging (BLI) was longitudinally performed at day 1, 7, 14 28 transplantation track survival proliferation transplanted cells. At 28, mice killed grafts explanted detect teratoma formation. The results demonstrated that iPSC-derivates iPSC-CMs survived receipts. Both proliferated dramatically transplantation, while only slight increase BLI signals observed iPSC-CM detected both mice, but not ones. In vitro study showed long-term existence cells during differentiation. Furthermore, when these passaged feeder layers as undifferentiated they would recover iPSC-like colonies, indicating cause iPSC's tumourigenicity. Our indicates exclusion by screening addition lineage-specific therapeutic iPSCs.