Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3‐dioxygenase1

• DOI: 10.1111/jcmm.12534
• Publication Date: 2015-03-18T05:00:59Z
• Authors: Rita Romani; Irene Pirisinu; Mario Calvitti; Maria Teresa Pall...; Marco Gargaro; Giovanni Bistoni; Carmine Vacca; Alessandro Di Mic...; Ciriana Orabona; Jessica Rosati; Matteo Pirro; Stefano Giovagnoli; Davide Matino; Paolo Prontera; Gabriella Rosi; Ursula Grohmann; Vincenzo N. Talesa; Emilio Donti; Paolo Puccetti; Francesca Fallarino
• Keywords: Amniotic epithelial cells; Indoleamine 2,3-dioxygenase; Amniotic stem cells

Abstract Although human amniotic fluid does contain different populations of foetal‐derived stem cells, scanty information is available on the stemness and potential immunomodulatory activity in vitro expanded, cells. By means a methodology unrequiring immune selection, we isolated characterized cell types from second‐trimester samples (human HASC s). Of those populations, one was by fast doubling time, cells were thus designated as fHASC s. Cells maintained their original phenotype under prolonged passaging, they able to originate embryoid bodies. Moreover, s exhibited regulatory properties when treated with interferon ( IFN )‐γ, including induction enzyme indoleamine 2,3‐dioxygenase 1 IDO 1). On coculture peripheral blood mononuclear ‐γ–treated caused significantly decreased T‐cell proliferation increased frequency CD 4 + 25 FOXP 3 T Both effects required an intact function contact‐independent. An unprecedented finding our study that purified vesicles abundantly expressed functional protein, endowed ‐like function. In vivo , capable immunoregulatory function, promoting allograft survival mouse model allogeneic skin transplantation. This concurrent expansion Foxp3 graft‐draining lymph nodes recipient mice. Thus s, or thereof, may represent novel opportunity for maneuvers both .