The role of TGF‐β1/Smad2/3 pathway in platelet‐rich plasma in retarding intervertebral disc degeneration

• DOI: 10.1111/jcmm.12847
• Publication Date: 2016-04-10T16:03:19Z
• Authors: Huilin Yang; Chenxi Yuan; Chunshen Wu; Jiale Qian; Qing Shi; Xuefeng Li; Xuesong Zhu; Jun Zou
• Keywords: Aggrecan; Intervertebral Disc; Matrix (chemical analysis)

Abstract Recent studies have suggested that platelet‐rich plasma ( PRP ) injections are an effective way to retard intervertebral disc degeneration, but the mechanism of action is unclear. Activated platelets release some growth factors, such as transforming factor‐β1 TGF ‐β1), which positively modulate extracellular matrix nucleus pulposus cells. The purpose this study was explore underlying ‐mediated inhibition degeneration. In in vitro study, we found proliferation cells greatly enhanced with 2.5% treatment. ‐β1 concentration much higher after administration effectively increased collagen II, aggrecan and sox‐9 mRNA levels decreased X levels. However, Western blotting demonstrated specifically inhibiting signalling could significantly prevent pulpous cellular expression Smad2/3 protein. a rabbit magnetic resonance imaging revealed significant recovery signal intensity discs injection group compared very low control groups. Histologically, plus inhibitor had lower II than group. These results high content retarded degeneration vivo . Inhibiting ‐β1/Smad2/3 pathway by inactivating down‐regulating matrix. Therefore, might play critical role ability