MiR‐9 is involved in TGF‐β1‐induced lung cancer cell invasion and adhesion by targeting SOX7

• DOI: 10.1111/jcmm.13120
• Publication Date: 2017-03-07T09:46:41Z
• Authors: Lichun Han; Wei Wang; Wei Ding; Lijian Zhang
• Keywords: Matrigel; Gentamicin protection assay

Abstract MicroRNA (miR)‐9 plays different roles in cancer types. Here, we investigated the role of miR‐9 non‐small‐cell lung ( NSCLC ) cell invasion and adhesion vitro explored whether was involved transforming growth factor‐beta 1 TGF ‐β1)‐induced by targeting SOX 7. The expression 7 human tissues lines examined reverse transcription‐quantitative polymerase chain reaction. Gain‐of‐function loss‐of‐function experiments were performed on A549 HCC 827 cells to investigate effect presence or absence ‐β1. Transwell–Matrigel assay used examine abilities. Luciferase reporter determine a direct target miR‐9. We found up‐regulated down‐regulated lines. Moreover, negatively correlated with expression. knockdown overexpression could suppress ‐β1‐induced adhesion. directly targets 3′ untranslated region 7, protein ‐β1 induced cells. up‐regulation led enhanced adhesion; however, these effects be attenuated overexpression. concluded that . contributed