Extracellular vesicles do not contribute to higher circulating levels of soluble LRP1 in idiopathic dilated cardiomyopathy

• DOI: 10.1111/jcmm.13211
• Publication Date: 2017-05-30T04:56:36Z
• Authors: Santiago Roura; Carolina Gálvez‐M...; David de Gonzalo‐...; Ana Gámez Valero; Paloma Gastelurrutia; Elena Revuelta‐López; Cristina Prat‐Vidal; Carolina Soler‐Bo...; Aida Llucià‐Valld...; Isaac Perea‐Gil; Oriol Iborra‐Egea; Francesc E. Borràs; Josep Lupón; Vicenta Llorente‐...; Antoni Bayes‐Genis
• Keywords: LRP1; Nanoparticle tracking analysis; Dilated Cardiomyopathy; Extracellular vesicles; Pathogenesis

Abstract Idiopathic dilated cardiomyopathy ( IDCM ) is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low‐density lipoprotein receptor‐related protein 1 LRP 1) has been linked to the underlying molecular basis disease. This study compared circulating levels soluble sLRP in patients healthy controls elucidated whether exported out myocardium through extracellular vesicles EV s) gain better understanding pathogenesis α chain expression was analysed samples collected from left ventricles explanted hearts using immunohistochemistry. concentrations were determined platelet‐free plasma by enzyme‐linked immunosorbent assay. Plasma‐derived s extracted size‐exclusion chromatography SEC characterized nanoparticle tracking analysis cryo‐transmission electron microscopy. The distributions vesicular CD 9, 81) myocardial (caveolin‐3) proteins assessed fractions flow cytometry. preferably localized vessels control myocardium. Circulating increased patients. 9‐ 81‐positive enriched with membrane expected size morphology isolated both groups. not present these fractions, which also positive for caveolin‐3. increase may be clinically valuable. Although do contribute higher , comprehensive content would provide further insights into search novel markers.