Atractylenolide II reverses the influence of lncRNA XIST/miR‐30a‐5p/ROR1 axis on chemo‐resistance of colorectal cancer cells

• DOI: 10.1111/jcmm.14148
• Publication Date: 2019-03-25T11:45:10Z
• Authors: Ruijuan Zhang; Zhijun Wang; Qianyun Yu; Jun Shen; Wenji He; Dongqing Zhou; Qingqing Yu; Jiawei Fan; Shurong Gao; Lihong Duan
• Keywords: XIST

This investigation was conducted to elucidate whether atractylenolide II could reverse the role of lncRNA XIST/miR-30a-5p/ROR1 axis in modulating chemosensitivity colorectal cancer cells. We totally collected 294 pairs tissues and adjacent normal also purchased cell lines human embryonic kidney line. 5-fluorouracil, cisplatin, mitomycin adriamycin were designated as chemotherapies for lines, atractylenolides arranged Chinese drug. The expressions XIST, miR-30a-5p ROR1 quantified with aid qRT-PCR or Western blot, luciferase reporter gene assay implemented determine relationships among ROR1. Our results demonstrated that XIST dramatically up-regulated, yet expression down-regulated within (P < 0.05). overexpressed ROR1, well under-expressed miR-30a-5p, inclined promote viability proliferation cells under influence chemo drugs In addition, directly target acted targeted molecule miR-30a-5p. Interestingly, not only switched but significantly intensified Finally, discovered slow down conclusion, be deemed pivotal markers underlying cancer, administration might improve chemotherapeutic efficacy cancer.