Anti‐angiogenic effect of quercetin and its 8‐methyl pentamethyl ether derivative in human microvascular endothelial cells

• DOI: 10.1111/jcmm.14455
• Publication Date: 2019-08-01T13:31:05Z
• Authors: Gabriella Lupo; Maria Teresa Cambria; Melania Olivieri; Concetta Rocco; Nunzia Caporarello; Anna Longo; Guido Zanghì; Mario Salmeri; Mario C. Foti; Carmelina Daniela...
• Keywords: Ex vivo

Abstract Angiogenesis is involved in many pathological states such as progression of tumours, retinopathy prematurity and diabetic retinopathy. The latter a more complex complication which neurodegeneration plays significant role leading cause blindness. vascular endothelial growth factor (VEGF) powerful pro‐angiogenic that acts through three tyrosine kinase receptors (VEGFR‐1, VEGFR‐2 VEGFR‐3). In this work we studied the anti‐angiogenic effect quercetin (Q) some its derivates human microvascular cells, blood retinal barrier model, after stimulation with VEGF‐A. We found permethylated form Q, namely 8MQPM, than simple potent inhibitor angiogenesis both vitro ex vivo. Our results showed these compounds inhibited cell viability migration disrupted formation microvessels rabbit aortic ring. addition Q significantly 8MQPM caused recoveries or completely re‐establish transendothelial electrical resistance (TEER) to control values suppressed activation VEGFR2 downstream signalling molecules AKT, extracellular signal‐regulated kinase, c‐Jun N‐terminal kinase. Taken together, data suggest might have an important contrast angiogenesis‐related diseases.