LIPH promotes metastasis by enriching stem‐like cells in triple‐negative breast cancer

• DOI: 10.1111/jcmm.15549
• Publication Date: 2020-07-03T02:29:30Z
• Authors: Yixiao Zhang; Xudong Zhu; Xinbo Qiao; Xi Gu; Jinqi Xue; Yanshuo Han; Lisha Sun; Meizi Cui; Caigang Liu
• Keywords: Adult; G2 Phase; 0303 health sciences; Epithelial-Mesenchymal Transition; Adolescent; Apoptosis; Triple Negative Breast Neoplasms; Original Articles; Cell Cycle Checkpoints; Lipase; Middle Aged; 3. Good health; Young Adult; 03 medical and health sciences; Hyaluronan Receptors; Oxygen Consumption; Cell Line, Tumor; Lymphatic Metastasis; Neoplastic Stem Cells; Humans; Female; Cell Division; Aged; Cell Proliferation

Abstract Lipase member H (LIPH), a novel of the triglyceride lipase family. The clinical implications its expression in breast cancer are still unclear. Therefore, this study, we investigated associations between LIPH and tumorigenic behaviours 144 triple‐negative (TNBC) patients. ratio mammosphere‐forming ability CD44+/CD24− stem‐like cells were tested. role cell migration invasion was also evaluated. In addition, effect silencing on mitochondrial respiration determined using Seahorse assay. Finally, protein via tandem mass tag‐based spectrometry Western blotting. We found that associated with metastasis lymph nodes distant organs ( P = 0.025), resulting poor survival among patients 0.027). knockdown significantly decreased both CD44 + /CD24 − their ability. promoted apoptosis, arrested cycle G2/M phase, mitigated oxidation‐related oxygen consumption rate mitochondria, reduced metabolism. inhibited adhesion tumour enhanced epithelial‐mesenchymal transition. Tandem spectrometric analysis presented 68 proteins differentially expressed LIPH‐silenced LIPH‐mediated modulation depended integrin‐related CAPN2 paxillin signalling. Overall, our findings provided strong evidence up‐regulation stemness TNBC cells. targeting is potentially viable strategy for preventing TNBC.