Quantitative gingival crevicular fluid proteome in health and periodontal disease using stable isotope chemistries and mass spectrometry

• DOI: 10.1111/jcpe.12262
• Publication Date: 2014-04-16T15:12:44Z
• Authors: Leandro G. Carneiro; Hesham Nouh; Erdjan Salih
• Keywords: Proteome; Tandem mass tag; Dental plaque

Application of quantitative stable isotope-labelling chemistries and mass spectrometry (MS) to determine alterations in gingival crevicular fluid (GCF) proteome periodontal disease.Quantitative GCF from 40 healthy individuals versus patients with disease was established using 320 samples reagents, ICAT mTRAQ, MS technology validated by enzyme-linked immunosorbent methods.We have identified 238 distinct proteins which 180 were quantified both additional 26 32 that found only or patients. In addition, 42 pathogenic bacterial 11 yeast quantified. The data highlighted a series not previously large-scale approaches relevance disease, such as host-derived Ig alpha-2 chain C, Kallikrein-4, S100-A9, transmembrane proteinase 13, peptidase S1 domain, several collagen types proteins, e.g. formamidase, leucine aminopeptidase virulence factor OMP85.The innovative analytical provided detailed novel changes host microbial derived proteomes study defined 50 16 significantly elevated most significant potential for application the clinical arena disease.