C‐reactive protein perturbs alveolar bone homeostasis: An experimental study of periodontitis and diabetes in the rat

• DOI: 10.1111/jcpe.13667
• Publication Date: 2022-05-30T07:34:43Z
• Authors: Mengjiao Zhou; Xiaohui Xu; Jie Li; Jie Zhou; Yao He; Ziqi Chen; Shan Liu; Duanjing Chen; Han Li; Guangyue Li; Jiao Huang; Gangyi Yang; Tingwei Zhang; Jinlin Song
• Keywords: Lipopolysaccharides; 0303 health sciences; Alveolar Bone Loss; X-Ray Microtomography; Alkaline Phosphatase; Streptozocin; Rats; 3. Good health; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; C-Reactive Protein; Glucose; Osteogenesis; Diabetes Mellitus; Animals; Homeostasis; Humans; Periodontitis; Proto-Oncogene Proteins c-akt

Abstract Aim To explore the role of C‐reactive protein (CRP) in periodontitis and diabetes its mechanism alveolar bone homeostasis. Materials Methods In vivo, normal, Crp knockout (KO) rats were randomly divided into control, diabetes, periodontitis, groups, respectively. The model was established using a high‐fat diet combined with streptozotocin injection. by ligature lipopolysaccharide (LPS) Alveolar bones analysed micro‐computed tomography, histology, immunohistochemistry. vitro, human periodontal ligament cells (hPDLCs) treated LPS high glucose. CRP knockdown lentivirus or overexpression adenovirus PI3K/AKT signalling inhibitor agonist used to regulatory osteogenesis osteoclastogenesis hPDLCs, as evidenced alkaline phosphatase staining, Western blot, quantitative polymerase chain reaction. Results KO decreased loss expression levels osteoclastogenic markers, while increasing osteogenic markers. constrained promoting hPDLCs via under glucose pro‐inflammatory conditions. Conclusions inhibits promotes diabetic conditions, thus perturbing