High‐yield nanovesicles extruded from dental follicle stem cells promote the regeneration of periodontal tissues as an alternative of exosomes

• DOI: 10.1111/jcpe.14036
• Publication Date: 2024-07-02T02:22:32Z
• Authors: Lu Liang; Limeiting Wang; Zhenhui Liao; Liya Ma; Pinwen Wang; Junjie Zhao; Jinyan Wu; Hefeng Yang
• Keywords: Regenerative Medicine; Dental follicle

Abstract Aim To identify an optimized strategy for the large‐scale production of nanovesicles (NVs) that preserve biological properties exosomes (EXOs) use in periodontal regeneration. Materials and Methods NVs from dental follicle stem cells (DFSCs) were prepared through extrusion, EXOs DFSCs isolated. The yield both extruded (eNVs) quantified protein concentration particle number analyses. Their pro‐migration, pro‐proliferation pro‐osteogenesis capacities compared subsequently vitro. Additionally, proteomics analysis was conducted. further evaluate regeneration potential eNVs EXOs, they incorporated into collagen sponges transplanted defects rats. In vivo imaging H&E staining utilized to verify their biodistribution safety. Micro‐Computed Tomography histological performed examine tissues. Results nearly 40 times higher than EXOs. Interestingly, vitro experiments indicated pro‐migration abilities superior, comparable More importantly, exhibited regenerative similar Conclusions Extrusion has proven be efficient method generating numerous with replace